Apolipoprotein M (ApoM) is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. ApoM is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions.
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:8000
Western Blot: 0.2ug/ml. Preliminary experiments gave an approx 50kD band in human kidney and liver lysates after antibody staining. Currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kD according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kD band was successfully blocked by incubation with the immunizing peptide.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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