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Apolipoprotein M (ApoM, G3a, HSPC336, MGC22400, NG20, NG20-like protein)

Apolipoprotein M (ApoM, G3a, HSPC336, MGC22400, NG20, NG20-like protein)

Cat no: A2299-42F

Supplier: United States Biological
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Apolipoprotein M (ApoM) is a member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. It is positively related to leptin. It is mainly expressed in liver and kidney. ApoM is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. It is important for the formation of prebeta-HDL and cholesterol efflux to HDL, and thereby inhibits formation of atherosclerotic lesions. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16000 Western Blot: 0.3-1ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Human Placenta lysates Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2299-42F
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by affinity chromatography.
References: 1. Karlsson H, Lindqvist H, Tagesson C, Lindahl M Characterization of apolipoprotein M isoforms in low-density lipoprotein J Proteome Res. 2006 Oct;5(10):2685-90 PMID: 17022639
Additional info: Recognizes human Apolipoprotein M at ~26kD. Species sequence homology: mouse, rat.

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