Apoptosis Inducing Factor, Mouse, Control Peptide \n(AIF)

Control Peptide for A1059-80A (antiserum) and A1059-80 (affinity purified)\n\n20-aa peptide from mouse AIF.\n\nMitochondria play a pivotal role in the regulation of programmed cell death or apoptosis. Apoptosis is driven by two classes of specialized proteases known as caspases (Cysteine aspartase). The initiator caspases can be activated by self-cleavage. The effector caspases are then activated in an amplification cascade. Several key participants are released from the mitochondria that regulate apoptosis. The first such factor (Cytochrome-C) to be described binds to a cytoplasmic scaffolding protein called Apaf-1. Binding of the mitochondria factor allows Apaf-1 to form a ternary complex with, and activate, the initiator pro-caspase-9. Active caspase-9 then turns on downstream effector caspases, initiating apoptosis. Another mitochondrial-derived factor, termed Apoptosis inducing factor (AIF), has been identified. AIF is sufficient to induce apoptosis of isolated nuclei. AIF (mouse 612 aa, rat 613 aa, and human 612 aa, chromosome Xq25-q26) is a flavoprotein of ~57kD. It normally resides in the mitochondrial but translocates to the nucleus. AIF induces mitochondrial to release Cytochrome-c and caspase-9. It has been found in liver and many other tissues.\n\nMouse and human AIF are 92% homologous. The C-terminus of AIF shares significant homology with several bacterial ferredoxins, and the N-terminus has two mitochondrial localization signal. The N-terminal 1-101aa are cleaved to produce mature AIF.\n\nApplications:\nSuitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 50-100ng of control peptide/well\nAntibody Blocking: 5-10ug\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0906-92C

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Highly purified

References

(1) Susin SA et al (1999) Nature 397, 441-445; Ohsakaya S et al (2000) Gene Acc. # AB041723; Joza N et al (2001) Nature 410, 549-554; Yu S-W et al (2002) Science 297, 259-263

Additional Info

95% conserved in rat and 61% in human AIF. No significant sequence homology is detected with other ferredoxins