logo
logo
APP-C99-tGFP U2OS Stable Cell line: Division-Arrested

Cat no: P30702-DA

APP-C99-tGFP U2OS Stable Cell line: Division-Arrested

Alzheimer disease (AD) is characterized by brain depositions of the beta amyloid (Beta-A). The Beta-A is the amyloid precursor protein (APP) digestion product, which is released from the cell after Beta--secretase and Gamma-secretase proteolysis. A novel recombinant cell line has been developed for screening inhibitors for Gamma-secretase activity via APP processing pathway.Cell Line Characterization:APP-C99 U2Os cells allow to perform assays to evaluate the endogenous Gamma-secretase proteolityc process in living cells. This cell line has been validated using different inhibitory compounds for Gamma-secretase (positive controls) and Beta-secretase activity (negative controls). High content analysis of Gamma-secretase activity has been designed to be performed using an epifluorescent imaging system to acquire and analyze images and to quantify the fluorescent vesicles into the citoplasm.The results obtained during the assay validation indicate that the pharmacological inhibition of Gamma-secretase implicated in AD is a valid strategy for drug screening and these models are appropriate to monitor the disease process in vivo in bioimaging systems.

Prices direct from B-Bridge International, Inc.

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P30702-DA

Size

2 x 10^6 cells

Read more on Supplier website

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...