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APP, Phosphorylated (Thr668) (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1)

Cat no: A2275-86Q

APP, Phosphorylated (Thr668) (Amyloid beta A4 Protein, ABPP, APPI, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, CVAP, PreA4, Protease Nexin-II, PN-II, A4, AD1)

Amyloid beta (A4) Precursor Protein (APP) is a ubiquitously expressed transmembrane protein that is sequentially cleaved by beta-secretase and gamma-secretase to release extracellular peptides such as the b-amyloid peptides, which are deposited in the brain in Alzheimer's disease. APP exists in several isoforms ranging from 100-140kD, and is phosphorylated on various residues within the extracellular and cytoplasmic domains, effecting the proteolytic processing and secretion of the protein. Threonine 668 can be phosphorylated by cyclin-dependent kinase 5 (cdk5), GSK-3b and JNK, and may play an important role in neurite outgrowth of differentiating neurons.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nCAD cells transfected with wild type vs. T668A mutant APP\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2275-86Q

Size

25ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Selkoe, DJ. et al. Trends Cell. Biol. 8(11), 447 (1998). 2.Tarr, PE. et al. J. Biol. Chem. 277(19), 16798 (2002).

Additional Info

Recognizes human APP when phosphorylated at Thr668. Species Crossreactivity: mouse, rat, and frog.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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