APRIL, ED2 (a Proliferation Inducing Ligand, TALL-2, TNF and ApoL-related Leukocyte-expressed Ligand 2, TRDL-1a, TNF-related Death Ligand 1a, TNFSF13)

Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identi?ed by several groups and designated APRIL (for a proliferation-inducing ligand), TALL-2 (for TNF-and ApoL-related Leukocyte-expressed Ligand 2), and TRDL-1a (for TNF related death ligand 1a) in human and mouse (1-4). Two receptors for APRIL were recently identi?ed and designated TACI and BCMA (5-7). APRIL stimulates B and T cell proliferation, triggers humoral immune responses, activates NF-kB, and induces cell death (1-7). APRIL and its close relative of BlyS and their receptors BCMA and TACI are involved in diseases of autoimmunity and cancer (8,9).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution: \nWestern Blot: 1-4ug/ml\nImmunohistochemistry: 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJ9000: Jurkat cell lysate\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A2299-99

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hahne, M., Kataoka, T., Schroter, M., et al., APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J. Exp. Med.188(6: 1185-90 (1998). 2. Shu, H.B., Hu, W.H., Johnson, H.. TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J. Leukoc. Biol. 65(5): 680-3 (1999). 3. Kelly, K., Manos, E., Jensen, G., et al., APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 60(4): 1021-7 (2000). 4. Yu, G., Boone, T., Delaney, J., et al., APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat. Immunol. 1(3): 252-6 (2000). 5. Wu, Y., Bressette, D., Carrell, J.A., et al., Tumor necrosis factor (TNF) receptor superfamily member TACI is a high af?nity receptor for TNF family members APRIL and BLyS. J. Biol. Chem. 275(45): 35478-85 (2000). 6. Marsters, S.A., Yan, M., Pitti, R.M., et al., Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr. Biol. 10(13): 785-8 (2000). 7. Rennert, P., Schneider, P., Cachero, T.G., et al., A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth. J. Exp. Med. 192(11): 1677-84 (2000). 8. Gross, J.A., Johnston J., Mudri, S., et al., TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease. Nature 404: 995-9 (2000). 9. Ware, C.F., APRIL and BAFF connect autoimmunity and cancer. J. Exp. Med. 192(11): F35-8 (2000).

Additional Info

Recognizes APRIL at ~27kD. Species Crossreactivity: mouse, rat