Aquaporin 3 (AQP3, GLIP)\n

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the C-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. AQP3 (GLIP, Glycerol-transporting integral protein; 285aa; 31.4kD) has a wide tissue distribution. AQP3 may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It is also slightly permeable to urea and glycerol. AQP3 may play an important role in gastrointestinal tract water transport and in glycerol metabolism. In the kidney it is predominantly expressed in the inner medulla. AQP families of proteins are predicted to contain six transmembrane domains. The N and C-termini are predicted to be cytoplasmic.\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\nRecommended Dilutions:\nWestern Blot: 1:1000-1:5000 using ECL. \nELISA: 1:100,000. Using 50-100ng control peptide/well.\nOptimal dilutions to be determined by the researcher.\nRecommended Control Peptide:\nA3000-11G: Aquaporin 3, Rat, Control Peptide (AQP3, GLIP).\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A3000-11D

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Ishibashi K et al (1994) Proc Natl Acad Sci, 91, 6269-6273; Echevarria M et al (1994) ) Proc Natl Acad Sci, 91, 10997-1100; Ma, TA et al (1994) J Biol. Chem. 269, 21845-21849; 2. Ecelbarger, CA et al (1995) Am. J. Physiol. 269, F663-F672\nWebsites: http://www.nature.com/labinvest/journal/v82/n11/full/3780560a.html and IF frozen human samples:\nhttp://www.jhc.org/cgi/reprint/47/10/1275.pdf, \nhttp://www.nature.com/jid/journal/v128/n3/full/5701047a.html

Additional Info

Recognizes rat AQP3.