Aquaporin 4 (AQP4, Aquaporin-4, AQP-4, WCH4, MIWC, Mercury-insensitive Water Channel)

Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells. The gene of AQP4 is mapped to 18q11.2-q12.1. Similar to other aquaporins, the AQP4 gene is composed of 4 exons encoding 127, 55, 27, and 92aa separated by introns of 0.8, 0.3, and 5.2kb. Unlike other aquaporins, an alternative coding initiation sequence (designated exon 0) was located 2.7kb upstream of exon 1. When spliced together, M1 and the subsequent 10aa are encoded by exon 0; the next 11aa and M23 are encoded by exon 1. AQP4 is expressed in astrocytes and is upregulated by direct insult to the central nervous system. AQP4 is the predominant water channel in the brain and has an important role in brain water homeostasis. It is abundant in mammalian brain and is concentrated in astrocytic foot processes at the blood-brain barrier.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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