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Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

Aquaporin 4 (AQP4, WCH4, MIWC, Mercury-insensitive Water Channel)

Cat no: A3000-14B

Supplier: United States Biological
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Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporins". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP4 (MIWC, mercury-insensitive water channel) is expressed in brain and in other tissues as well. Aquaporin 4 is located in various tissues including the gastro-intestinal tract, kidney and brain. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:1000-1:2000 Western Blot: 1:1000-1:10,000; Predicted MW: 38.8kD Immunoprecipitation: 1:1000 -1:10,000 Immunohistochemistry (paraffin): 1:100; Perform heat mediated antigen retrieval with sodium citrate buffer pH 6.0. Immunohistochemistry (frozen): 1:100; Use snap-frozen, acetone-fixed tissue only. Optimal dilutions to be determined by the researcher. Positive Control: Kidney tissue Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A3000-14B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 50ug
Form: Supplied as a liquid in PBS, pH 7.4, 1% BSA., 0.09% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Badis, B., et al., J. Physiol. Paris 95: 271-275 (2001). 2. Nagy, G., et al., Pathol. Oncol. Res. 8(2): 115-124 (2002).
Additional info: Recognizes an epitope within the cytoplasmic domain of water-specific channel Aquaporin 4. Species Crossreactivity: human. Does not crossreact with rat or mouse.

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