Aquaporin 5 (AQP5)

Water is a critical component of all living cells. Tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by "aquaporin". Genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a trans- poson-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an aa substitution that inhibits targeting of MIP to the cell membrane. AQP5 (282aa) is found in several epithelial tissues (salivary and lachrymal glands, corneal epithelium in the eye and lung). It is implicated in generation of saliva, tears, and pulmonary secretions. AQP families of proteins are predicted to contain six transmembrane domains. Rat-AQP5 is a 265 amino acid transmembrane protein.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 (ECL).\nELISA: 1:10,000-1:50,000 Coat with 1ug/ml Control Peptide.\nOptimal dilutions to be determined by the researcher.\n\nControl peptide:\nA3000-23: Aquaporin 5, Rat, Control Peptide (AQP5)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A3000-21

Size

50ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Serum

Isotype

IgG1

References

1. Raina, S. et al., 1995, J. Biol. Chem. 270:1908-1912. 2. Lee MD et al., 1996, JBC 271, 8599-8604. 3. Krane C.M., et al., 1999, Mamm. Genome 10, 498-505.

Additional Info

Reconizes rat Aquaporin 5 (AQP5). No significant homology is seen with other AQPs or any other protein. Species Sequence Homology: mouse: 100%; human: 80%; sheep: 76%.