Arachis hypogaea (PNA, Peanut Agglutinin)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. \n\nLectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.\n\nArachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen") is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells.пїЅThe presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies. \n\nBiological Activity:\n< 1ug/ml will agglutinate human erythrocytes neuraminidase treatment of cells.\n\nCarbohydrate Specificity:\nTerminal beta-galactose\n\nInhibitory Carbohydrate:\nLactose > beta-galactose\n\nQuality Control:\nSDS-PAGE\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile buffer. Aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A3305

Size

5mg

Applications

IHC

Form

Supplied as a lyophilized powder from 0.01M phosphate-0.15M sodium chloride, pH 7.3. Reconstitute with buffer to a concentration of 1mg/ml.

References

1. Lotan, R., et al., J. Biol. Chem. 250(21): 8518 (1975). 2. Novogrodsky, N., et al., J. Immunol. 115: 1243 (1975). 3. Cooper, H.S., Human Pathology 15: 904-906 (1984). 4. Bird, G.N. & Wingham, J., Scand. J. Haematol. 8: 307-308 (1971). 5. Moller, P., Virchows Arch. 396: 313-317 (1982). 6. Vierbuchen, M. & Klein, P.J. Laboratory Inv. 48(2): 181 (1983). 7. Ree, H.J. & Hsu, S-M., Cancer 51: 1631 (1983). 8. Okada, M., et al., J. Immunol. 141: 1543-1549 (1988).

Alternative Names

peanut