Produced by hyperimmunizing goats with highly purified lectins. Specific antibodies are isolated by affinity chromatography on lectin-agarose columns, and then optimally labeled with biotin. Biotinylated anti-lectins can be used as intermediates to localize lectin receptors in tissues or on cells, or to follow lectin migration in neuronal transport. In some applications, biotinylated anti-lectins used in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.