Arachis hypogaea (PNA, Peanut Agglutinin) (HRP)

Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. \n\nLectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.\n\nArachis hypogaea (PNA, Peanut Agglutinin), 110kD, is composed of four identical subunits of approximately 27kD each, is galactose-specific, with strong anti-T-antigen activity. T-antigen is present in many glycoconjugates such as M and N blood groups, gangliosides, and many other soluble and membrane-associated glycoproteins and glycolipids. PNA does not agglutinate normal human erythrocytes, but strongly agglutinates neuraminidase-treated red blood cells.пїЅThe presence of Ca++ can enhance the binding of PNA to receptors. Asialo GM1 ganglioside is also thought to be a major cell surface receptor for PNA. PNA is useful in normal and tumor tissue determination for transitional mucosa malignancies. \n\nActivity:\n<1ug/ml will agglutinate human erythrocytes neuraminidase treatment of cells.\n\nInhibitory Carbohydrate: Lactose > beta-galactose\n\nCarbohydrate Specificity: Terminal beta-galactose\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.пїЅLabeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A3305-25

Size

500ug

Form

Supplied as a liquid in PBS, pH 7.3. Labeled with horseradish peroxidase (HRP).

References

1. Cooper, H.S. (1984) Human Pathology 15: 904-906. 2. Moller, P. (1982) Virchows Arch. 396: 313-317. 3. Vierbuchen, M. and Klein, P.J. (1983) Laboratory Inv. 48(2): 181. 4. Ree, H.J. and Hsy, S. (1983) Cancer 51: 1,631.

Alternative Names

peanut