Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells (agsNSC) are isolated from the hippocampus of adult Arctic Ground Squirrels following hibernation. Both the isolated cells and the whole animals are tolerant of ischemic insult and reperfusion. Using these cells, researchers can identify potentially novel proteins and genes key to neuronal tolerance, neuroprotection and neurogenesis. Creative Bioarray's agsNSC can be used to screen drugs, genes and proteins for protective changes caused by oxygen and/or glucose deprivation. These agsNSC could identify future targets for novel stroke therapeutics. As stem cells, these cells can be used in transplantation studies using rodent stroke models to investigate tolerance to ischemic injury.