ARF, p19

In humans, the multiple tumor suppressor 1 locus (MTS1) encodes two unrelated genes, p16INK4a and p19ARF. Although both act as cell proliferation inhibitors, their mechanisms of action are different. p19ARF works as part of a p53-dependent pathway to counter uncontrolled proliferation and oncogenic signals. Mice lacking the p19ARF gene rapidly develop a broad spectrum of tumors. This result indicates that p19ARF is an important tumor suppressor. In addition, p19ARF helps prevent transforming signals from various oncoproteins via the p53 regulatory loop (3). These studies and others display the importance of p19ARF in cell cycle control and tumor suppression.\n\nBy Western blot, this antibody recognizes IVT murine p19ARF and a clear band of the same size in CTLL2 cells. It detects a specific band in WT mouse embryo fibroblasts which is not present in p19ARF-null MEFs (1). The antibody can be competed from recognizing p19ARF using the antigenic peptide at 10ug/ml.\n\nRecommended Dilution: \nWestern Blot: 0.5-1ug/ml (ECL)\nImmunohistochemistry: Not determined\nELISA (indirect): 1:5,000

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SPECIFICATIONS

Catalog Number

A3330-09A

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Antisera was eluted with 100 mM glycine, pH 2.5, and dialyzed against PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by affinity chromatography.

Isotype

IgG

References

1. Radfar, A., et al. P19ARF induces p53-dependent apoptosis during abelson virus-mediated pre-B cell transformation. Proc Natl Acad Sci USA. 95(22): 13194-99, 1998. 2. Larsen, C.J. Alternative protein p19ARF: a genuine tumor suppressor gene. Bull. Cancer. 85(4): 304-6, 1998. 3. de Stanchina, E., et al. E1A signaling to p53 involves the p19ARF tumor suppressor. Genes Dev. 12(15): 2434-42, 1998.\n4. Zindy, F., et al. Myc signaling via the ARF tumor suppressor regulates p53-dependent apoptosis and immortalization. Genes Dev. 12(15): 2424-33, 1998.\nGeneral References:\n1.Grateful acknowledgments to M. Serrano and I. Palmero, National Center of Biotechnology, Madrid.

Additional Info

Specific for p19ARF. Species crossreactivity: Reacts with mouse p19ARF. Does not react with rat and human.