ADP-Ribosylation Factor 6 (ARF6) is a GTP-binding protein that functions as a regulator of cytoskeleton remodeling and endocytic recycling. ARF6 is an allosteric activator of cholera toxin catalytic subunit, an ADP-ribosyltransferase. It may also be involved in the modulation of vesicle budding and uncoating with the Golgi apparatus.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:500
Optimal dilutions to be determined by the researcher.
Positive Control:
NIH/3T3 Cell Lysate
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.