Home  >  Products  >  ARFGAP3 (ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526)

ARFGAP3 (ADP-ribosylation factor GTPase activating protein 3, ARFGAP1, RP1-47A17.3, ADP-ribosylation factor GTPase activating protein 1, OTTHUMP00000028526)

Cat no: A3335-01B


Supplier: United States Biological
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The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP-ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A3335-01B
Reactivities: Human, Mouse, Canine, Rabbit
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Liu X, Zhang C, Xing G, Chen Q, He F. Functional characterization of novel human ARFGAP3. FEBS Lett 2001 Feb 9;490(1-2):79-83.
Additional info: Recognizes ARFGAP3 at ~55-60kD in mouse brain extracts. Species Crossreactivity: Canine, mouse and rat.

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