ARG1 (Arginase 1, AI type I arginase, arginase 1 liver)

Arginase 1 (ARG1) is a 35-40kD member of the arginase family of enzymes. It is expressed in multiple cell types, including erythrocytes, hepatocytes, neutrophils, smooth muscle and macrophages. ARG1 demonstrates two distinct functions: in the hepatocyte cytoplasm, it catalyzes the conversion of arginine to ornithine and urea, while in multiple cells, it degrades arginine, thus indirectly downregulating NO synthase (NOS) activity by depriving this enzyme of its substrate. Human AGR1 is 322aa in length. Its enzyme region comprises aa9-309 and contains two Mn atoms. ARG1 is modestly active as a monomer, but highly active as a 105kD homotrimer. Trimerization is promoted by nitrosylation of Cys303, creating a regulatory feedback loop with NOS. There are two isoform variants, one that shows an eight aa insertion after Gln43, and another that shows a deletion of aa204-289. Full-length human ARG1 shares 87% aa identity with mouse and rat ARG1. \n\nApplications: \nSuitable for use in ELISA, Immunoprecicpitation and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nDirect ELISA: 0.5-1ug/ml. The detection limist is ~0.3ng/well.\nImmunoprecipitation: Used to immunoprecipitate recombinant human ARG1 from E. coli lysates.\nWestern Blot: 0.25ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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