Arginase Assay Kit, BioAssay(TM)

Arginase (L-arginine ureohydrolase EC 3.5.3.1) is present in mammals and plants. In humans, arginase is expressed predominantly in the liver, and to lesser degrees in breast, kidney, testes, salivary glands, epidermis and erythrocytes. Arginase catalyzes the conversion of arginine to ornithine and urea, completing the last step in the urea cycle. Arginase activity is a key diagnostic indicator. Increased levels of arginase activity in blood have been associated with liver damage. Hyperargininemia due to arginase deficiency is an inherited autosomal recessive disease.\n\nSimple, direct and automation-ready procedures for measuring arginase activity in biological samples are highly desirable in Research and Drug Discovery. Arginase assay kit provides a sensitive and convenient method for arginase activity determination. The method utilizes a chromogen that forms a colored complex specifically with urea produced in the arginase reaction. The intensity of the color is directly proportional to the arginase activity in the sample.\n\nKey Features:\nSensitive and accurate. Detection limit: 0.3U/L for 2 hour arginase reaction in 96-well assay format.\nSimple and high-throughput. The procedure involves incubation of the provided substrate with the sample in a microplate followed by the addition of the coloring reagent. Can be readily automated as a high-throughput assay for thousands of samples per day.\n\nApplications:\nDirect Assays: arginase activity in enzyme preparations, serum, plasma, tissue culture etc; Drug Discovery/Pharmacology: effects of drugs on arginase activity.\n\nKit Components:\n*A3340-11A: Arginine Buffer, pH 9.5, 1x2ml \nA3340-11B: Mn Solution, 1x1ml\nA3340-11C: Reagent A, 1x25ml \nA3340-11D: Reagent B, 1x25ml\n*A3340-11E: Urea standard, 50mg/dL, 1x1ml\n\nStorage and Stability:\nStorage and Stability:\nStore *A3340-11A and *A3340-11E at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nPrecautions: \nReagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents.

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