Arginase-I (Liver Type) (EC 3.5.3.1, L-arginine aminohydrolase)

Arginase is an enzyme that hydrolyzes Larginine to L-ornithine and urea in the urea cycle. Two forms of arginase exists which are designed as arginase I and arginase II. Liver-type arginase I is expressed primarily in the liver and to some extend in the erythrocytes. Arginase II is expressed in many extrahepatic tissues, such as brain, spinal cord, kidney, small intestine and mammary gland. Although arginase I and arginase II have similar enzyme activities, they have different pI, immunological reactivity and are encoded by different genes. Human arginase I is a 35kD protein circulating in blood probably as a homotrimer. Circulating liver-type arginase was clinically used as a liver specific marker which may reflect not only early occurrence of liver injury but also early termination of liver injury. The measurement of liver-type arginase is clinically applicable for monitoring conditions of patients with liver disorders or pre- and postoperative conditions of patients who received partial hepatectomy with quicker normalization in comparison with aminotransferases (ALT and AST). Recently, arginase I gene was found to be one of the most prominent among astma genes. In situ hybridization demonstrated marked staining of arginase I in submucosal inflammatory lesions and arginase activity increased in allergen challenged lungs. Finally, it was found that both arginase I was the most significantly up-regulated protein in the murine spinal cord during experimental autoimmune encephalomyelitis. The results indicated that arginase I played important roles in autoimmune inflammation in the central nervous system.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A3340-04

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with 100ul sterile ddH2O.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Perozich J, Hempel J, Morris Biochim Biophys Acta Jan;1382(1):23-37 (1998) 2. Orellana MS, Lopez V, Uribe E, Fuentes M, Salas M, Carvajal N. Arch Biochem Biophys. 2002 Jul 15;403(2):155-9. 3. Porembska Z, Zabek J, Grabon W, Rahden-Staron I, Baranczyk-Kuzma A. Clin Chim Acta. 2001 Mar;305(1-2):157-65. 4. Ikemoto M, Ishida A, Tsunekawa S, Ozawa K, Kasai Y, Totani M, Ueda K. Clin Chem. 1993 May;39(5):794-9. 5. Lavulo LT, Emig FA, Ash DE Arch Biochem Biophys Mar;399(1):49-55 (2002) 6. Morris SM, Bhamidipati D, Kepka-Lenhart D Gene Jul;193(2):157-61 (1997).

Additional Info

Recognizes human liver-type aginase.