ARL3, CT (ADP-ribosylation Factor-like Protein 3, ARFL3)

ADP-ribosylation factors (ARFs) are low molecular weight GTP-binding proteins belonging to the RAS superfamily. The predicted 182aa ARL3 (ADP-ribosylation like factor) protein shares 97% amino acid identity with rat ARLl3 and 43% identity with human ARF1. Like the ARFs, ARL3 has a glycine at position 2, the site of N myristoylation, and lacks cysteine residues near the C terminus, which are found in other members of the RAS family. Northern blot analysis detected a 1-kb ARL3 transcript in all tissues tested, with highest expression in heart and lung, and lower expression in brain, liver, kidney, ovary, and testis. A 5.5-kb transcript was also detected in most tissues, with highest expression in brain. Immunoblot analysis detected ARL3 in human tumor cell lines but not in normal rodent cells. Although ARL3 binds GTP, it is devoid of activity in the cholera toxin-dependent ADP-ribosylation of Gs, and is therefore classified as an ARF-like protein.\n\nApplications: \nSuitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000\nWestern Blot: 1:100-1:500\nImmunohistochemistry: 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A3570-85A

Size

200ul

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Cavenagh, M.M., et al., J. Biol. Chem. 269(29):18937-18942 (1994). 2. Adams, M.D., et al., Nature 377(6547 Suppl), 3-174 (1995). 3. Kim, H.S., Cytogenet. Cell Genet. 83(3-4), 246 (1998). 4. Wistow, G., et al., Mol. Vis. 8, 196-204 (2002).

Additional Info

Recognizes human ARL3.