ARP2

Cat no: A3574-91

Supplier: United States Biological

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Actin nucleation, the formation of new actin filaments from existing filaments, affects actin filament structure during cell motility, division and intracellular trafficking. An important actin nucleation protein complex is the highly conserved ARP2/3 complex, consisting of ARP2, ARP3 and ARPC1-5. The ARP2/3 complex promotes branching of an existing actin filament and formation of a daughter filament following activation by nucleation-promoting factors, such as WASP/WAVE or cortactin (1). The formation of podosomes, small cellular projections that degrade the extracellular matrix, is enhanced by ARP2/3 complex action. ARP2/3 competes with caldesmon, an actin binding protein shown to negatively affect podosome formation (2). The ARP2/3 complex (along with N-WASP) regulates nuclear actin filament nucleation and controls actin polymerization during transcription (3). Applications: Suitable for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Western blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	A3574-91
Reactivities:	Human, Mouse, Rat, Drosophila/Arthropod, Non-Human Primate
Hosts:	Rabbit
Applications:	Western Blot
Size:	100ul
Form:	Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Serum
Alternative names:	(Actin Like Protein 2, Actin-related Protein 2, ACTR2)
References:	1.Goley, E.D. and Welch, M.D. (2006) Nat. Rev. Mol. Cell Biol. 7, 713-726. 2. Morita, T. et al. (2007) J. Biol. Chem. 282, 8454-8463. 3. Yoo, Y. et al. (2007) J. Biol. Chem. 282, 7616-7623.
Additional info:	Recognizes endogenous levels of total human ARP2 protein. Species crossreactivity: mouse, rat, monkey, hamster and D.melanogaster.

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ARP2

Cat no: A3574-91

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