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ARPC2 (Actin Related Protein 2/3 Complex Subunit 2 34kD, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 Protein Compex Subunit 34)

ARPC2 (Actin Related Protein 2/3 Complex Subunit 2 34kD, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 Protein Compex Subunit 34)

Cat no: A3576A

Supplier: United States Biological
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The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. Positive Control: Hela and Human Muscle lysates. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000. Western Blot: 0.1-0.5ug/ml. Detects a band of approximately 34kD in Hela and Human Muscle lysates. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A3576A
Reactivities: Human
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in TBS, pH 7.2, 0.5% BSA.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Welch MD et al. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 138:375-84 (1997).
Additional info: This antibody is expected to recognize both reported isoforms as represented by NP_005722 and NP_690601. Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse, Rat and Zebrafish (90% identity with immunogen) due to sequence homology.

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