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ARPC3 (Actin Related Protein 2/3 Complex Subunit 3 (21kD), ARP2/3 Protein Complex Subunit p21, ARC21, p21-Arc)

ARPC3 (Actin Related Protein 2/3 Complex Subunit 3 (21kD), ARP2/3 Protein Complex Subunit p21, ARC21, p21-Arc)

Cat no: A3576-02

Supplier: United States Biological
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This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p21 subunit, has yet to be determined. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000. Western Blot: 1-3ug/ml. Band observed just below 20kD marker in Hela, Human Brain and Mouse Brain lysates (predicted MW of 20kD according to NP_005710). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A3576-02
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ. The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol. 1997 Jul 28;138(2):375-84. PMID: 9230079
Additional info: Species Crossreactivity: Tested: Human, Mouse. Expected from sequence similarity: Human, Mouse, Rat

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