Artemin is a disulfide-linked homodimeric neurotrophic factor structurally related to GDNF, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. Artemin, GDNF, Persephin and Neurturin all signal through a multicomponent receptor system, composed of RET (receptor tyrosine kinase) and one of the four GFRa (a1-a4) receptors. Artemin prefers the receptor GFRa3-RET, but will use other receptors as an alternative. Artemin supports the survival of all peripheral ganglia such as sympathetic, neural crest and placodally derived sensory neurons, and dopaminergic midbrain neurons. The functional human Artemin ligand is a disulfide-linked homodimer, of two 12.0kD polypeptide monomers. Each monomer contains seven conserved cysteine residues, one of which is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Recombinant human Artemin is 24.2kD, disulfide-linked homodimer formedby two identical 113 amino acid subunits.
AA Sequence:
AGGPGSRARA AGARGCRLRS QLVPVRALGL GHRSDELVRF RFCSGSCRRA RSPHDLSLAS LLGAGALRPP PGSRPVSQPC CRPTRYEAVS FMDVNSTWRT VDRLSATACG CLG
Biological Activity:
The ED50 as determined by its ability to stimulate the proliferation of human SH-SY5Y cells is 2.0-5.0ng/ml. The biological activity was also determined by its ability to promote neuronal survival and neurite outgrowth on dorsal root ganglion neurons.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute with ddH2O, carrier protein. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin:
USA
Quick Search
Products 
