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Arylsulfatase B, Recombinant, Human (ARSB)

Arylsulfatase B, Recombinant, Human (ARSB)

Cat no: A3596-50

Supplier: United States Biological
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ARSB is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3). Activity: Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS). The specific activity, measured under the described conditions, is > 3,000 pmoles/min/ug. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Catalogue number: A3596-50
Size: 10ug
Form: Supplied in a 0.2um filtered liquid in 25 mM Tris, 0.15 M NaCl, pH 7.5
Purity: ~ 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin: < 1EU per 1ug.
References: 1. Peters, C. et al., 1990, J. Biol. Chem. 265:3374. 2. O
Additional info: Recognizes human Arylsulfatase B.

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