Arylsulfatase G, Recombinant, Mouse (ARSG)

Cat no: A3596-62

Supplier: United States Biological

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ARSG is a member of the aryl sulfatase family of enzymes with a high degree of amino acid sequence similarity to ARSA and ARSB. ARSG mRNA is expressed in a ubiquitous manner, being detected in most adult and embryonic tissues of the mouse (1). Recombinant mouse Arylsulfatase G encoded by the Arsg gene (rmARSG; amino acid residues 17-525; Accession # NP_082986) (1) was expressed with an N-terminal signal sequence and a C-terminal 10X His tag in a Chinese Hamster Ovary cell line. Calculated molecular mass of 55kD and migrates as a 6 kD band in SDS-PAGE under reducing conditions. Activity: Measured by its ability to hydrolyze the substrate 4-nitrocatechol sulfate (PNCS). Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	A3596-62
Size:	10ug
Form:	Supplied as a liquid in 25mM Tris, 0.15M sodium chlroide, pH 7.5.
Purity:	(same/more than) 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: (same/less than) 1EU/1ug.
References:	1. Ferrante, F. et al., 2002, Eur. J. Hum. Genetics. 10:813-818.

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Arylsulfatase G, Recombinant, Mouse (ARSG)

Cat no: A3596-62

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