AS3MT catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (AdoMet) to trivalent arsenical and may play a role in arsenic metabolism.
Source:
Recombinant corresponding to aa1-375 from human AS3MT, fused to His-tag at N-terminus, expressed in E.coli.
Molecular Weight:
~44.3kD (399aa) confirmed by MALDI-TOF
AA Sequence:
MGSSHHHHHH SSGLVPRGSH MGSHMAALRD AEIQKDVQTY YGQVLKRSAD LQTNGCVTTA RPVPKHIREA LQNVHEEVAL RYYGCGLVIP EHLENCWILD LGSGSGRDCY VLSQLVGEKG HVTGIDMTKG QVEVAEKYLD YHMEKYGFQA SNVTFIHGYI EKLGEAGIKN ESHDIVVSNC VINLVPDKQQ VLQEAYRVLK HGGELYFSDV YTSLELPEEI RTHKVLWGEC LGGALYWKEL AVLAQKIGFC PPRLVTANLI TIQNKELERV IGDCRFVSAT FRLFKHSKTG PTKRCQVIYN GGITGHEKEL MFDANFTFKE GEIVEVDEET AAILKNSRFA QDFLIRPIGE KLPTSGGCSA LELKDIITDP FKLAEESDSM KSRCVPDAAG GCCGTKKSC
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.