Ascorbic Acid Assay Kit, BioAssay(TM)

Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. This Ascorbic Acid Assay Kit provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample. This kit is suitable for the determination of ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates.\n\nSample size: 20ul \n\nLinear Detection Range: \nColorimetric Assay: 6 to 1000uM ascorbic acid\nFluorimetric Assay: 1 to 100uM ascorbic acid\n\nApplications:\nAssays: Ascorbic acid in biological samples such as serum, plasma, urine, saliva, milk, and tissue/cell lysates\nDrug Discovery/Pharmacology: Effects of drugs on ascorbic acid metabolism\n\nKit Components:\nA3774-61A: Enzyme Mix, 1x120ul\nA3774-61B: Standard, 1x400ul. 10mM Ascorbic acid\nA3774-61C: Assay Buffer, 1x10ml\nA3774-61D: Dye Reagent, 1x120ul\n\nMaterials Required But Not Provided:\nOptical density plate reader\nFluorescence plate reader.\nPipeting devices\nCentrifuge tubes\nClear flat-bottom uncoated 96-well plates\nBlack flat-bottom uncoated 96-well plates\n\nStorage and Stability:\nStore all kit components at --20 degrees C. Kit is stable for 6 months.

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SPECIFICATIONS

Catalog Number

A3774-61

Size

100Tests

References

1. Baker, W.L. and Lowe, T. (1985). Sensitive ascorbic acid assay for the analysis of pharmaceutical products and fruit juices. Analyst 110: 1189-1191.\n2. Chung, W.Y. et al (2001). Plasma ascorbic acid: measurement, stability and clinical utility revisited. Clin Biochem. 34: 623-627.\n3. Arya, S.P. et al (2002). A new method for the ascorbic acid assay using iron(II)-pyridine-2,6-dicarboxylic acid complex. Ann Chim. 92: 1159-1164.