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ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)

ASH2 (ASH2L1, ASH2L2, Absent, Small, Homeotic-Like 2, Trithorax protein ASH2)

Cat no: A3782-10F

Supplier: United States Biological
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The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 on Lys4 and functions as a transcriptional co-ac-tivator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3 and MLL4, all of which methylate his-tone H3 on Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common structural subunits WDR5, RBBP5 and ASH2L (2-6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 on Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1 histone methyltransferase complexes play a critical role in leukemogenesis (6). Applications: Suitable for use in RIA, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000 Western Blot: 1:2000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A3782-10F
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
P type: Mab
Purity: Purified
References: 1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7, 2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8, 3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36, 4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31, 5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28, 6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.
Additional info: Recongnizes endogenous levels of human all known isoforms of the ASH2L protein. Species crossreactivity: mouse, rat, monkey. Sequence Homology: melanogaster.

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