Home  >  Products  >  ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)
ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)

ASIALOGLYCOPROTEIN RECEPTOR (ASGPR)

Cat no: A8510

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42kD) which mediates binding, internalisation and degradation of extracellular glycoproteins that have exposed terminal galactose residues. The receptor is expressed on the surface of the hepatocytes in a polar manner, i.e. it is present on the sinusoidal and lateral plasma membranes but not on the bile canalicular membrane. The mammalian hepatic ASGPR mediates the endocytosis and degradation of serum proteins from which terminal sialic residues have been removed. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50 Western Blot: 1:50 Detects a band at ~42kD. A non-reduced sample treatment and SDS-PAGE followed by blotting on nitrocellulose or PDFM is recommended. Block with 10% non-fat dry milk. Immunohistochemistry (frozen): 1:50 Acetone-fixed ELISA: 20ug/ml using 100ul/well Optimal dilutions to be determined by the researcher. Recommended Positive Control: Rat hepatocytes Recommended Negative Control: Rat Kidney, pancreas, small intestine, colon Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A8510
Reactivities: Human, Rat
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Shimada, M et al; A monoclonal antibody to rat asialoglycoprotein receptor that recognizes an epitope specific to its major subunit. Hepatol Res 2003, 26: 55 2. Mizuno, M et al; Monoclonal antibodies identifying antigens on distinct domains of rat hepatocytes. Liver 1987, 7: 251 3. Hyodo, I et al; Distribution of asioalogycoprotein receptor in human hepatocellular carcinoma. Liver 1993, 13: 80 4. Mizuno, M et al: Development of a monoclonal antibody identifying an antigen which is segregated to the sinusoidal and lateral plasma membranes of rat hepatocytes. Gastroenterol Japan 1986, 21: 238
Additional info: Recognizes a subunit-specific epitope on RHL-1 of rat ASGPR. Species crossreactivity: human.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave