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Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)

Atg3 (Autophagy-related Protein 3, APG3, ATG3 Autophagy Related 3 Homolog (S. cerevisiae), APG3-like, AUT1, Protein PC3-96)

Cat no: A2298-74W

Supplier: United States Biological
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GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy. SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. Belongs to the ATG3 family. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 1:50-1:3000 Immunofluorescence: 1:50-1:3000 Western Blot: 1:50-1:3000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2298-74W
Reactivities: Human, Mouse, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute with 100ul of sterile ddH2O.
P type: Pab
Isotype: IgG
Purity: Serum
Alternative names: Autophagy-related E2-like conjugation enzyme ATG3
References: 1. Tanida I, et al. J. Biol. Chem. 277:13739-13744(2002). 2. Ota T, et al. Nat. Genet. 36:40-45(2004). 3. Rush J, et al. Nat. Biotechnol. 23:94-101(2005).
Additional info: Recognizes human ATG3 at ~37kD. Species Crossreactivity: rat and mouse

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