Mammalian cells undergoing apoptosis express high levels of APG5L (or ASP) protein relatively late in the apoptotic process. APG5L protein contains a high number of acidic residues, a potential glycosylation site, and potential casein kinase II, protein kinase C, and tyrosine kinase sites. May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
Source:
Recombinant protein corresponding to APG5L, fused to T7 tag at N-terminal expressed in E.coli.
Molecular Weight:
~31.6kD
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.