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Atg7, CT (APG7, APG7-like, Apoptosis-specific protein, Autophagy protein 7, hAPG7, Ubiquitin-Activation Enzyme E1-like Protein)

Atg7, CT (APG7, APG7-like, Apoptosis-specific protein, Autophagy protein 7, hAPG7, Ubiquitin-Activation Enzyme E1-like Protein)

Cat no: A2298-74R3

Supplier: United States Biological
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In mammalian cells, Autophagy-related protein 7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Studies indicate that caspase-8 may alter ATG7 levels and thus the ATG7 program of autophagic cell death. ATG7 is evolutionally conserved and exhibits high homology within mammalian species. It expressed in wide range of tissues including kidney, liver, bone marrow and lymph nodes. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:10-1:50 Immunohistochemistry (Frozen): 1:10-1:50 Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1903-30U: IgG, H&L, X-adsorbed (HRP) ShxRb I1904-40J: IgG, Fc (HRP) Pab Gt xRb I1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A2298-74R3
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: Ubiquitin-Activation Enzyme E1-like Protein, ATG7, APG7
Additional info: Recognizes human Autophagy-related protein 7, a 78kD cytoplasmic protein. Species Crossreactivity: mouse

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