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Atg9A (Autophagy Related 9 Homolog A, APG9L1, FLJ22169, MGD3208)

Atg9A (Autophagy Related 9 Homolog A, APG9L1, FLJ22169, MGD3208)

Cat no: A3935-50C

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:3000 Western Blot: 1:100-1:3000 Immunohistochemistry: 1:100-1:3000 Optimal dilutions to be determined by the researcher. Cellular Localization: Membrane; multi-pass membrane protein. Preautophagosomal structure; preautophagosomal structure membrane; multi-pass membrane protein. Note: Pre-autophagosomal and other perivacuolar punctate structures. The proper trafficking of ATG9 between the pre-autophagosomal structure and the other punctate structures requires ATG2, ATG18, ATG23, the ATG1-ATG13 complex and the phosphatidylinositol 3-kinase complex I. Belongs to the ATG9 family. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A3935-50C
Reactivities: Human, Rat
Hosts: Rabbit
Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a lyophilized powder. Reconstitute in 100ul of sterile water. Centrifuge to remove any insoluble material.
P type: Pab
Purity: Serum
Alternative names: Autophagy-related Protein 9A, APG9-like 1, Cytoplasm to Vacuole Targeting Protein 7, Autophagy-related Protein 9, APG9A
References: 1. Ota T, e al. Nat. Genet. 36:40-45(2004). ; 2. Delaveau T.T.D, et al. Yeast 12:1587-1592(1996). ; 3. Jacq C, et al. Nature 387:75-78(1997). ; 4. Tsukada M., Ohsumi Y. FEBS Lett. 333:169-174(1993). ; 5. Harding T.M, et al. J. Cell Biol. 131:591-602(1995). ; 6. Harding T.M, et al. J. Biol. Chem. 271:17621-17624(1996). ; 7. Lang T, et al. J. Bacteriol. 182:2125-2133(2000). ; 8. Noda T, et al. J. Cell Biol. 148:465-480(2000). ; 9. Kim H, et al. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006). ; 10. Tucker K.A, et al. J. Biol. Chem. 278:48445-48452(2003).
Additional info: IHC, WB and ELISA confirmed the specificity for ATG9A (APG9L1) . Species Crossreactivity: Human, rat. Other species not yet tested.

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