Copper is essential for human health and copper imbalance is a key factor in the aetiology and pathology of several neurodegenerative diseases. Copper uptake into cells is thought to be mediated by the plasma membrane protein CTR1. Metallochaperones also bind copper and target it to specific destinations within the cell. ATOX1 (HAH1) transfers copper to the copper-ATPases. Copper-transporting ATPases (Cu-ATPases) ATP7A and ATP7B are evolutionarily conserved polytopic membrane proteins with essential roles in human physiology. The Cu-ATPases are expressed in most tissues, and their transport activity is crucial for central nervous system development, liver function, connective tissue formation, and many other physiological processes. These proteins have a dual role in cells, namely to provide sufficient amounts of essential intracellular copper and to mediate the excretion of excess of intracellular copper.
Applications:
Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Paraffin): Fixed in Bouin's solution for 20 hours at 4 degrees C and paraffin embedded. Endogenous peroxidase activity was blocked with 0.1% H2O2 in methanol (15min).
Immunohistochemistry (Frozen): Postfixed overnight in paraformaldehyde (4 degrees C), followed by incubation in 25% sucrose in PBS + 0.02% NaN3 (4 degrees C). Sections (12um) were cut on a cryostat and collected on gelatin-coated slides
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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