ATPase alpha 1, Na, K

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic alpha subunit and a beta subunit (reviewed in 1). Several phosphorylation sites have been identified for the alpha1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the alpha subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A4000-52B

Size

100ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.

P Type

Pab

Purity

Purified by affinity chromatography.

References

1. Therien, A.G. and Blostein, R. (2000) Am. J. Physiol. Cell Physiol. 279, C541-566. 2. Feraille, E. et al. (1999) Mol. Biol. Cell 10, 2847-2859. 3. Fisone, G. et al. (1994) J. Biol. Chem. 269, 9368-9373. 4. Feschenko, M.S. and Sweadner, K.J. (1995) J. Biol. Chem. 270, 14072-14077. 5. Beguin, P. et al. (1994) J. Biol. Chem. 269, 24437-24445. 6. Yingst, D.R. et al. (2004) Am. J. Physiol. Renal Physiol. 287, F713-721. 7. Al-Khalili, L. et al. (2004) J. Biol. Chem. 279, 25211-25218. 8. Tian, J. et al. (2006) Mol. Biol. Cell 17, 317-326. 9. Liang, M. et al. (2006) J. Biol. Chem. 281, 19709-19719.

Additional Info

Recognizes endogeneous levels of total human Na, K-ATPase a1. Species crossreactivity: mouse, rat, monkey, hamster