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ATPase alpha, Na, K

ATPase alpha, Na, K

Cat no: A4000-51Y

Supplier: United States Biological
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Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry (Fr). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4000-51Y
Reactivities: Human, Rat, Canine, Chicken/Bird, Porcine, Sheep
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 200ul
Form: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Ascites
References: This product has been used in:Ball WJ et al. Isolation and characterization of monoclonal antibodies to (Na+ + K+)-ATPase. Biochim Biophys Acta 719:413-23 (1982). Malik B et al. Identification of the amino acids comprising a surface-exposed epitope within the nucleotide-binding domain of the Na+,K(+)-ATPase using a random peptide library. Protein Sci 2:2103-11 (1993). Abbott A & Ball WJ The epitope for the inhibitory antibody M7-PB-E9 contains Ser-646 and Asp-652 of the sheep Na+,K(+)-ATPase alpha-subunit. Biochemistry 32:3511-8 (1993). Abbott A & Ball WJ The inhibitory monoclonal antibody M7-PB-E9 stabilizes E2 conformational states of Na+,K(+)-ATPase. Biochemistry 31:11236-43 (1992). Sweadner KJ Isozymes of the Na+/K+-ATPase. Biochim Biophys Acta 988:185-220 (1989).
Additional info: Detects sodium / potassium ATPase alpha.This antibody detects the alpha 3 isoform in rat only. Species Crossreactivity: Crossreacts with Human, Rat, bovine, canine, porcine, Sheep and Chicken.Not yet tested in other species.

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