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ATPase, Ca (ATP2B1, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium ATPase 1, PMCA1 ATPase)

ATPase, Ca (ATP2B1, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium ATPase 1, PMCA1 ATPase)

Cat no: A4000-50A2

Supplier: United States Biological
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The calcium pump of the plasma membrane, termed PMCA ATPase, pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the SERCA ATPase and the sodium/potassium pump. There are four different genes encoding PMCA ATPase and studies have revealed 20 isoforms of the pump generated by alternate splicing of the primary gene products. mRNA distribution studies show that gene products 1 and 4 are transcribed in most tissues, however, products 2 and 3 are more tissue specific. Transcription of the splicing variants has also been found to be tissue specific. In the pancreas, where insulin secretion is calcium dependent, the beta cells only express the 4b isoform, however the alpha and gamma cells express both 4a and 4b isoforms. Studies have also shown that different splice variants have different affinities for calcium and calmodulin. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Detects ~134kD and ~130kD proteins representing PMCA 1a and 1b ATPase from rat samples Optimal dilutions to be determined by the researcher. Localization: Cell Membrane; Multi-pass membrane protein Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4000-50A2
Reactivities: Human, Rat
Hosts: Rabbit
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS containing 1mg/ml BSA and 0.05% sodium azide
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1. iloteo AG et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-7 (1997). 2, Caride AJ et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J 316 (Pt 1):353-9 (1996).
Additional info: Recognizes rat and human PMCA 1a and 1b ATPase

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