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ATPase, Ca (Plasma Membrane 1, PMCA1)

ATPase, Ca (Plasma Membrane 1, PMCA1)

Cat no: A4000-50A6

Supplier: United States Biological
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The Ca(2+)-ATPases are a family of plasma membrane pumps encoded by at least 4 genes: ATP2B1 on chromosome 12q21; ATP2B2; ATP2B3 on Xq28; and ATP2B4 on 1q25. The proteins share 84% aa sequence identity and 76% nucleic acid sequence homology. The function of calcium-transporting ATPase found in different membranes is to lower cytoplasmic Ca(2+) concentration by pumping Ca(2+) to luminal or extracellular spaces. Mutations in a plasma membrane Ca(2+)-ATPase gene cause deafness in deafwaddler mice. Application: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 4ug/ml Immunohistochemistry (paraffin): 8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4000-50A6
Reactivities: Human, Mouse, Rat, Chicken/Bird, Rabbit
Hosts: Mouse
Applications: Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a lyophilized powder from 1.2% sodium acetate, 2% BSA, 0.01% sodium azide. Reconstitute with PBS.
P type: Mab
Isotype: IgG2a
Purity: Purified by immunoaffinity chromatography.
Additional info: Recognizes human PMCA. Species Crossreactivity: mouse, rat, chicken, rabbit.

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