ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) ELISA Kit

Cat no: ABIN429319

Supplier: Antibodies-online

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ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) ELISA Kit: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm

Catalogue number:	ABIN429319
Size:	96 tests
Accession:	P50992
Gene:	11945
Antigen:	ATP4b
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	0.156-10 ng/mL
Sample type:	Tissue Homogenate,Biological Fluids
Entrez gene:	11945

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ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b) ELISA Kit

Cat no: ABIN429319

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