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ATPase, Na, K

ATPase, Na, K

Cat no: A4000-86A

Supplier: United States Biological
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Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, whereas the beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase a1 subunit, in brain, is expressed in both neuronal and non-neuronal cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:100-1:500 ELISA: 1:1,000-1:6,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Reconstitute with sterile ddH2O, sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4000-86A
Reactivities: Canine
Hosts: Chicken/Bird
Applications: ELISA, Western Blot
Size: 1mg
Form: Supplied as a lyophilized powder from 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide. Reconstitute with 2ml of deionized water.
P type: Pab
Purity: Purified by Ion Exchange chromatography.
Additional info: Recognizes purified and partially purified ATPase, sodium, potassium (canine kidney). Reactive against the intact enzyme and enzyme subunits (alpha at 100kD and beta at 50kD) as well as glycosylated and non-glycosylated enzyme forms.

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