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ATPase PMCA4

ATPase PMCA4

Cat no: A4000-50D

Supplier: United States Biological
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Plasma membrane calcium-ATPase 4 (PMCA4) expression shows little variation across eight drug-treated cell lines and was found to be superior to GAPDH and HPRT1, commonly used reference genes. PMCA4 can be used as a reference gene for normalizing ABC transporter expression in a drug-resistant lung carcinoma cell line. PMCA4 is a good housekeeping gene for normalization of gene expression for polytopic membrane proteins including transporters and receptors. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:15,000 Western Blot: 1:1000 Immunohistochemistry (Frozen & Paraffin): 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: A4000-50D
Reactivities: Human, Rat, Porcine
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified
References: Filoteo, A.G. et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-23747 (1997). Caride, A.J. et al. Detection of isoform 4 of the plasma membrane calcium pump in human tissues by using isoform-specific monoclonal antibodies. Biochem J. 316:353-359 (1996).
Additional info: Recognizes human PMCA4 4 isoforms, 4a and 4b, at ~133 and 129kD, respectively. Species Crossreactivity: porcine and rat

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