Autophagy Protein Detection Kit, BioAssay(TM)

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles (3). Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4,5), is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors (6). PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity (5). LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome (7,8 and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression (9). However, the loss of both proteins results in embryonic lethality (10), suggesting that each protein possesses some unique and necessary functions.\n\nKit Components: \nA4200-05A: APG7 Antibody, 1x50ug\nA4200-05B: Beclin-1 Antibody, 1x50ug \nA4200-05C: LAMP1 Antibody, 1x50ug \nA4200-05D: LAMP2 Antibody, 1x50ug \nA4200-05E: PIST Antibody, 1x50ug \n\nStorage and Stability:\nStore at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nSource: \nRabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins. \n\nApplications: \nSuitable for use in Western Blot. A4200-05A, and A4200-05B are suitable for use in Immunocytochemistry and A4200-05E is suitable for use in Immunohistochemistry for detection of their resective proteins.

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SPECIFICATIONS

Catalog Number

A4200-05

Size

1Kit

Applications

WB

Reactivities

Hum, Mouse, Rat

Form

Each antibody is supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

Purity

Purified by immunoaffinity chromatography.

References

1. Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. 2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5. 3. Komatsu M, Waguri S, Ueno T, et al. Impairment of starvationinduced and constitutively autophagy in Atg7-deficient mice. J. Cell. Biol. 2005; 169:425-34. 4. Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96. 5. Kametaka S, Okano T, Ohsumi M, et al. Apg14p and Apg6/Vps30p form a protein complex essential for autophagy in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 1998; 273:22284-91. 6. Liang XH, Jackson S, Seaman M, et al. Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature 1999; 402:672-6. 7. Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95. 8. Tanaka Y, Guhde G, Suter A, et al. Accumulation of autophagic vacuoles and cardiopathy in LAMP-2-deficient mice. Nature 2000; 902-6. 9. Andrejewski N, Punnonen EL, Guhde G, et al. Normal lysosomal morphology and function in LAMP-1-deficient mice. J. Biol. Chem. 1999; 274:12692-701.10. Gamp AC, Tanaka Y, Lullmann-Rauch R, et al. LIMP-2/LGP85 deficiency causes ureteric pelvic junction obstruction, deafness, and peripheral neuropathy in mice. Hum. Mol. Genetic. 2003; 12:631-46.

Additional Info

Antibodies to recognize human APG7, Beclin-1, LAMP1, LAMP2 and PIST. Species crossreactivity: mouse and rat.