Aven, CT

Cat no: A4445-11M

Supplier: United States Biological

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Description
Additional Information

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a vari ety of diseases such as cancer (reviewed in 1). Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9 (2). It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients (3). Despite its predicted molecular weight, Aven often migrates at 55kD in SDS-PAGE. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml. See A4445-10A for control peptide. Immunohistochemistry: 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: Raji cell lysate Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Catalogue number:	A4445-11M
Reactivities:	Human
Hosts:	Rabbit
Applications:	Immunohistochemistry, Western Blot
Size:	100ug
Form:	Supplied as a liquid in PBS, 0.02% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified by immunoaffinity chromatography.
References:	1. Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. 2. Chau BN, Cheng EH-Y, Kerr DA, et al. Aven, a novel inhibitor of caspase activation. Binds Bcl-xL and Apaf-1. Mol. Cell 2000; 6:31-40. 3. Paydas S, Tanriverdi K, Yavuz S, et al. Survivin and aven: two distinct antiapoptotic signals in acute leukemias. Ann. Oncology 2003; 14:1045-50.
Additional info:	Recognizes human Aven.

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Aven, CT

Cat no: A4445-11M

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