Avidin D, Rhodamine (TMRITC) labeled, is made from ultrapure Avidin D and is optimally labeled using tetramethyl- rhodamine isothiocyanate (TMRITC). Rhodamine Avidin D has an absorption maximum at about 550nm and a maximum emission at about 575nm. This label produces a red color and is recommended for routine immunofluorescence applications. Rhodamine provides a second label to fluorescein and is especially useful in avoiding problems associated with endogenous tissue fluorescence. The lower quantum yield of rhodamine compared to fluorescein is compensated for by a reduction in photobleaching rate.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry: 5-30ug/ml
Optimal dilutions to be determined by the researcher.
Excitation:
550-560nm
Emission:
568-575nm
F/P (molar):
2.8
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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