Avidin (Egg White, 68kD Glycoprotein)

Avidin (Biotin Binding Protein) is a strongly basic glycoprotein synthesized in the hen oviduct and deposited in the albumen fraction of eggs at a ratio of 0.05% of the total protein content. It binds the water-soluble vitamin H (d-biotin) very tightly and specifically with a binding log constant of 15. Avidin is a tetrameric protein, composed of subunits of identical amino acid composition and sequence. Each monomer is glycosylated on Asn-17. \n\nThe high affinity constant of Avidin for Biotin has been widely used in molecular biology: affinity chromatography, molecular recognition and labelling, Enzyme Linked ImmunoSorbent Assay (ELISA), histochemistry and cytochemistry. An excellent review in the field has been written by Wilchek, M. and Bayer, E.A., eds. (1990), 'Avidin-Biotin Technology', Methods in Enzymology, Vol.184, Academic Press.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\n\n

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SPECIFICATIONS

Catalog Number

A4500

Size

10mg

References

Berger, M., and Wood, H.: Purification of the Subunits of Transcarboxylase by Affinity Chromatography on Avidin-Sepharose, J. Biol. Chem., 250, 927 (1975).\nFraenkel-Conrat, H., Snell, N., and Ducay, E.: Avidin. I. Isolation and Characterization of the Protein and Nucleic Acid, Arch. Biochem. Biophys., 39, 80 (1952).\nGreen, N.: Avidin. 3. The Nature of the Biotin-Binding Site, Biochem. J., 89, 599 (1963a).\nGreen, N.: Purification of Avidin, in Methods in Enzymology, XVIII, (McCormick, D., and Wright, L., eds.), Academic Press, NY, 414 (1970a).\nGreen, N.: The Molecular Weight of Avidin, Biochem. J., 92, 16c (1964).\nGreen, N.: A Spectrophotometric Assay for Avidin and Biotin Based on Binding of Dyes by Avidin, Biochem. J., 94, 23c (1965).\nMelamed, M., and Green, N.: Avidin. 2. Purification and Composition, Biochem. J., 89, 591 (1963).\nWei, R., and Wright, L.: Heat Stability of Avidin and Avidin-Biotin Complex and Influence of Ionic Strength on Affinity of Avidin for Biotin, Proc. Soc. Exp. Biol. Med., 117, 341 (1964).