Avidin is a glycosylated polypeptide chain having a molecular mass of 68kD and containg 4 subunits each with a binding site for biotin. Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).
Activity:
Greater than 11 U/mg, 1 unit binds 1microg biotin.
Endotoxin:
(same/less than) 0.1ng/ug (IEU/ug) of Avidin.
Application:
Avidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
Solubility:
It is recommended to reconstitute the lyophilized Avidin in sterile 18MOmega-cm H2O not less than 100microg/ml or more than 10mg/ml. solutions
Stability:
Avidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
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