Avidin (Egg White, 68kD Glycoprotein)

Avidin is a glycosylated polypeptide chain having a molecular mass of 68kD and containg 4 subunits each with a binding site for biotin. Avidin is purified by affinity chromatographic techniques. The purification procedure ensures minimal contamination by other proteins or DNA. The resulting high activity and purity of the product gives very low non-specific binding (NSB).\n\nActivity: \nGreater than 11 U/mg, 1 unit binds 1microg biotin.\n\nEndotoxin: \n(same/less than) 0.1ng/ug (IEU/ug) of Avidin.\n\nApplication: \nAvidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.\n\nSolubility: \nIt is recommended to reconstitute the lyophilized Avidin in sterile 18MOmega-cm H2O not less than 100microg/ml or more than 10mg/ml. solutions\n\nStability: \nAvidin although stable at 40C for 3 weeks, should be stored desiccated below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A4500-02

Size

5mg

Form

Supplied as a lyophilized powder.

Purity

Purified by affinity chromatographic techniques.

References

1. Differential recovery of biotinylated microbial proteins using monomeric or polymeric avidin.\n\nBiotechniques 2006 May;40(5):584, 586, 588\n\n2. Separation of cationic polymer particles and characterization of avidin-immobilized particles by capillary electrophoresis.\n\nElectrophoresis 2006 Mar;27(5-6):1031-40\n\n3. Avidin-biotin system pretargeting radioimmunoimaging and radioimmunotherapy and its application in mouse model of human colon carcinoma.\n\nWorld J Gastroenterol 2005 Oct 28;11(40):6288-94\n\n4. Recognition of oxidatively modified bases within the biotin-binding site of avidin.\n\nJ Mol Biol 2006 Mar 17;357(1):263-74\n\n5. Avidin, a potential biopesticide and synergist to Bacillus thuringiensis toxins against field crop insects.\n\nJ Econ Entomol 2005 Oct;98(5):1566-71\n\n6. Mediastinal node and diaphragmatic targeting after intracavitary injection of avidin/99mTc-blue-biotin-liposome system.\n\nJ Pharm Sci 2006 Jan;95(1):207-24