Axl, phosphorylated (Tyr779) (AXL Oncogene, AXL Receptor Tyrosine Kinase, AXL Transforming Gene, AXL Transforming Sequence/gene, Adhesion-related Kinase, Ark, Oncogene AXL, Tyrosine Protein Kinase Receptor, UFO, TYR07)

Axl (also known as ARK, UFO and TYR07) is a receptor tyrosine kinase that contains extracellular regions composed of two IgG-related domains linked to two fibronectin type III repeats. Gas6, a ligand for Axl, induces Axl phosphorylation on multiple tyrosine residues. Phosphorylation of Axl at Tyr779 is associated with the activation of PI 3-kinase. \n\nApplications:\nSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1ug/ml detects Axl in Wl38 human lung cell line conditioned media-treated A172 human glioblastoma cell line.\nImmunocytochemistry: 5-15ug/ml detects Axl in immersion fixed A172 human glioblastoma cell line with and without pervanadate co-staining with total Axl.\nImmunohistochemistry: 5-15ug/ml detects Axl in immersion fixed paraffin embedded sections of human stomach.\nOptimal dilution to be determined by researcher.\n\nRecommended Secondary Antibodies:\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb\nI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb\n \nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts