Often times, multiple samples will be included, differing in the amino acid sequence used to raise the immune sera. NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation (1). NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase (IKK) complex. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Five members of IKK family, IKKa, IKKb, IKKg, NAK/TBK/T2K, and IKKi contribute to the activation of NF-kB dependent gene expression. NAP1 (NA-associated protein 1) is a recently identified protein that interacts with NAK and IKKi. NAP1 induces oligomerization of NAK. NAK-NAP1 complex alone can activate NF-kB byphosphorylating Serine 536 of the p65 subunit of NF-kB.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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